Journal: Oncotarget

Article Title: Novel chemokine-like activities of histones in tumor metastasis

doi: 10.18632/oncotarget.11226

Figure Lengend Snippet: A-C. Histones (50 μg/ml, 24 hours) induced chemokine production and release as demonstrated with a Proteome Profiler™ Antibody Array in Hepa1-6 cells. D. Knockdown of NF-κB p65 and TLR4 (but not TLR2 and RAGE) in Hepa1-6 cells inhibited histone (50 μg/ml, 24 hours)-induced CCL9/10 release as demonstrated by ELISA assay (n=3, *, p<0.05 versus control shRNA group). E. Anti-CCL9/10 neutralizing antibody (1 mg/ml) partly inhibited histone (50 μg/ml, 24 hours)-induced Hepa1-6 cell migration (n=3, *, p<0.05).

Article Snippet: The production or release of chemokines was assayed using a Proteome ProfilerTM Antibody Chemokine Array Kit (#ARY020) from R&D Systems Inc. according to the manufacturer's instructions.

Techniques: Ab Array, Knockdown, Enzyme-linked Immunosorbent Assay, Control, shRNA, Migration

Journal: Oncotarget

Article Title: Novel chemokine-like activities of histones in tumor metastasis

doi: 10.18632/oncotarget.11226

Figure Lengend Snippet: A-B. Compared with the control group, TLR4 depletion (by using TLR4 −/− mice or TLR4 knockdown cells) or inhibition of histone release (by administration of 10 mg/kg heparin or 10 mg/kg H3 neutralizing antibody) limited the formation of lung metastasis (as shown in arrow) in mice based on tail vein injection of 3×10 6 Hepa1-6 cells (N=5 mice/group, *, p<0.05 versus control group). In contrast, control IgG (10 mg/kg) did not inhibit the formation of lung metastasis (B). C. Serum nucleosome levels were reduced after treatment with heparin in wildtype, but not in TLR4 −/− mice (N=5 mice/group, *, p<0.05 versus control group). D. Conceptual relationships between histone and tumor metastasis. Histone is a nuclear DAMP and can be released during cell injury or death. Once released, histone can promote cell migration and invasion through the TLR4-ERK-NF-κB pathway, which induces chemokine production and release.

Article Snippet: The production or release of chemokines was assayed using a Proteome ProfilerTM Antibody Chemokine Array Kit (#ARY020) from R&D Systems Inc. according to the manufacturer's instructions.

Techniques: Control, Knockdown, Inhibition, Injection, Migration

Journal: iScience

Article Title: Intermittent fasting induced ketogenesis inhibits mouse epithelial ovarian cancer by promoting antitumor T cell response

doi: 10.1016/j.isci.2023.107839

Figure Lengend Snippet:

Article Snippet: Ascites collected from RD and IF mice (n = 4/group) were pooled and diluted 1:3 and applied in the adipokine array (ARY-013, R&D systems, Minneapolis, MN, USA).

Techniques: Recombinant, Software

Journal: Nature Communications

Article Title: High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance

doi: 10.1038/s41467-020-20500-w

Figure Lengend Snippet: a Plasma and WAT cytokine array of mice that received CD63 + A33 + exosomes (L-Exo or H-Exo) for 14 days. b Fold change in H-Exo vs. L-Exo-induced plasma cytokine expression for all cytokines showing greater than two-fold change. Red bars show cytokines/factors known to be involved in insulin resistance. c TNF-α (left) and IL-6 (right) upregulation following treatment with H-Exo were confirmed by ELISA in plasma. Filled circle—PBS, filled triangle—L-Exo, and filled rectangle—H-Exo. d ITT performed on C57BL/6 mice that received exosomes via adoptive transfer for 14 days followed with or without macrophage depletion. Filled rectangle—macrophage-depleted mice treated with H-Exo; filled diamond—mice without macrophage depletion treated with PBS and circle—mice without macrophage depletion treated with H-Exo. e Glucose uptake assay performed on hepatocytes cultured with different concentrations of H-Exo (as indicated in the figure). f Glucose uptake assay performed on mouse hepatocytes supplemented with supernatant derived from macrophages cultured with nanoparticles derived from H-Exo total lipids (H-Exo Nano) and PC (34:2). g Supernatants from H-Exo-treated macrophages (monocytes+ 5 × 10 6 ) were preneutralized with anti-TNF-α and/or anti-IL-6 antibodies. Glucose uptake by hepatocytes cultured in the presence of preneutralized supernatant was estimated. Data are represented as the mean ± SD. One-way ANOVA with a Tukey post hoc test. * < 0.05; **** < 0.0001. Source data are provided as a Source Data file.

Article Snippet: Cytokines were analyzed with a Proteome Profiler Mouse XL Cytokine Array Kit (R&D Systems, ARY028) as per the manufacturer’s instructions.

Techniques: Clinical Proteomics, Expressing, Enzyme-linked Immunosorbent Assay, Adoptive Transfer Assay, Cell Culture, Derivative Assay

Journal: Journal of Cerebral Blood Flow & Metabolism

Article Title: Non-classical monocytes promote neurovascular repair in cerebral small vessel disease associated with microinfarctions via CX3CR1

doi: 10.1177/0271678X231183742

Figure Lengend Snippet: Non-classical monocytes promote vascular function in cSVD. (a) Representative LSCI showing the temporal changes in the CBF of WT C and MDP mice up to 7 days post-cSVD. Analysis of CBF (b) at baseline (c) 24 h, (d) 3 days and (e) 7 days post-cSVD, shown as ipsilateral/contralateral (I/C) ratio. (f) Representative images of the Proteome Profiler mouse angiogenesis array membranes profiling expression of proteins implicated in vascular remodeling in the serum of CX3CR1 GFP/+ C and MDP mice 3 days post-cSVD. Analysis of the expression (optical density) of (g) CD105, (h) NOV/CCN3, (i) PDGF-AA and (j) VEGF, (k) serpin E1, (l) serpin F1, (m) thrombospondin 2 and (n) TIMP-4 in the serum of CX3CR1 GFP/+ C and MDP mice. Data are boxplot with min/max (n = 4–5 animals/group; n = 4 dots/experimental condition). I/C ratio = 1 indicates similar pattern in ipsilateral and contralateral hemispheres. *P < 0.05/**P < 0.01/***P < 0.001/ **** P < 0.0001 compared to WT C mice or CX3CR1 GFP/+ C mice (unpaired two-tailed t -test). Statistical summary is provided in (Supplementary Material 2). D, days.

Article Snippet: Blood was collected and allowed to clot in Eppendorf tubes for 2 h at RT and centrifuged at 2000×g for 20 min. Supernatant was collected and processed in a membrane-based Proteome Profiler TM mouse angiogenesis array kit (R&D, ARY015), following manufacturer’s recommendations.

Techniques: Expressing, Two Tailed Test