Journal: Frontiers in Neurology

Article Title: Inhibition of Colony Stimulating Factor 1 Receptor Suppresses Neuroinflammation and Neonatal Hypoxic-Ischemic Brain Injury

doi: 10.3389/fneur.2021.607370

Figure Lengend Snippet: PLX3397 changes brain cytokine/chemokine profile in HI mice. (A) The total differentially expressed cytokines/chemokines in the brains of HI mice treated with PLX3397 or the vehicle. Brain tissues were collected at 48 h after HI induction and the protein levels were measured with a cytokine/chemokine profile ELISA array kit in which 111 factors were detected. n = 3 independent experiments. Each independent experiment needed 10 mice per group (5 males and 5 females). Unpaired two-tailed t -test. (B) The mRNA levels of representative differentially expressed factors related to regulation of chemotaxis (CCL2, CCL12), immune response (CD14, M-CSF, IL-7), and BBB integrity (ICAM-1, VEGF, and WISP-1). CX3CL1 was as a negative control that was not significantly changed in protein level between HI mice treated with PLX3397 or the vehicle (data not shown). Brain tissues were collected at 48 h after HI from HI mice treated with PLX3397 or the vehicle. n = 5 mice per group. Unpaired two-tailed t -test. Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01.

Article Snippet: Cytokines in the cerebral tissue lysates were measured using a mouse XL cytokine array kit (ARY028, R&D Systems).

Techniques: Enzyme-linked Immunosorbent Assay, Two Tailed Test, Chemotaxis Assay, Negative Control

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: E-Selectin-Dependent Inflammation and Lipolysis in Adipose Tissue Exacerbate Steatosis-to-NASH Progression via S100A8/9

doi: 10.1016/j.jcmgh.2021.08.002

Figure Lengend Snippet: Genetic deletion of the Sele gene reduces inflammation, lipolysis, and proinflammatory adipokine secretion in adipose tissue from HFD + AdCxcl1 -induced NASH. WT (n = 4–7) and Sele KO (n = 4–7) were subjected to 3-month HFD feeding plus Ad Cxcl1 to induce NASH. Sera and epididymal adipose tissues were collected and analyzed. ( A ) MPO and F4/80 immunostaining of adipose tissues. ( B , C , and E ) RT-qPCR of adipose tissues. ( D ) Determination of serum FFAs. ( F ) Determination of serum proinflammatory adipokines. ∗ P < .05.

Article Snippet: The Proteome Profiler Mouse Adipokine Array Kit (R&D Systems) was used for the determination of IGFBPs, resistin, ANGPTL3, dipeptidyl peptidase 4, leptin, and RBP4 in serum samples pooled per group.

Techniques: Immunostaining, Quantitative RT-PCR

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: E-Selectin-Dependent Inflammation and Lipolysis in Adipose Tissue Exacerbate Steatosis-to-NASH Progression via S100A8/9

doi: 10.1016/j.jcmgh.2021.08.002

Figure Lengend Snippet: Inhibition of S100A9 ameliorates liver damage and inflammation in NASH by lowering lipolysis and adipokines in adipose tissue. WT mice were subjected to 4-month HFD feeding, followed by injection of Ad Cxcl1 and treatment with 2 different doses of PAQ: 10 μg/kg (PAQ10) (n = 7) or 20 μg/kg (PAQ20) (n = 6), or vehicle control (VC) (n = 5). Serum, liver, and adipose tissues were collected and analyzed. ( A ) Schematics for the administration of PAQ to HFD +Ad Cxcl1 -challenged mice. ( B ) Serum ALT and AST levels. ( C and D ) RT-qPCR of liver tissues. ( E and F ) RT-qPCR of adipose tissues. ( G and H ) Determination of serum FFAs and serum resistin. ( I ) RT-qPCR analyses of differentiated preadipocytes exposed to vehicle control or S100A8/A9. ( J ) Schematic illustration of the proposed role of E-selectin and S100A8/A9 in the liver and adipose tissue during neutrophil-induced NASH. ∗ P < .05.

Article Snippet: The Proteome Profiler Mouse Adipokine Array Kit (R&D Systems) was used for the determination of IGFBPs, resistin, ANGPTL3, dipeptidyl peptidase 4, leptin, and RBP4 in serum samples pooled per group.

Techniques: Inhibition, Injection, Control, Quantitative RT-PCR